Optimising chromatography strategies of antibody purification processes by mixed integer fractional programming techniques

The strategies employed in chromatography steps play a key role in downstream processes for monoclonal antibody (mAb) manufacture. This work addresses the integrated optimisation of chromatography step sequencing and column sizing in mAb purification processes. Chromatography sequencing decisions include the resin selection at each typical step, while the column sizing decisions include the number of columns, the column diameter and bed height, and number of cycles per batch. A mixed integer nonlinear programming (MINLP) model was developed and then reformulated as a mixed integer linear fractional programming (MILFP) model. A literature approach, the Dinkelbach algorithm, was adopted as the soluiopharmaceutical manufacturing rocesses Ab hromatography purification INLP ILFP tion method for the MILFP model. Finally, an industrially-relevant case study was investigated for the applicability of the proposed models and approaches. © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).